Journal: Journal of Veterinary Internal Medicine

Article Title: Can levamisole upregulate the equine cell‐mediated macrophage (M1) dendritic cell (DC1) T‐helper 1 (CD4 Th1) T‐cytotoxic (CD8) immune response in vitro?

doi: 10.1111/jvim.15404

Figure Lengend Snippet: Staining combinations used for immune phenotype analysis

Article Snippet: Cells were resuspended and stained with optimized concentrations of cell surface antibodies CD21 (BD557327), CD4 (Abd Serotec MCA1078F), CD8 (Abd Serotec MCA1080PE), CD14 (Wagner laboratory clone 105), CD172a (Kingfisher Biotech WS0567B‐100) and activation markers major histocompatibility complex (MHC) class II (Abd Serotec MCA1085F), CD86 (BD Biosciences 555 665), and FoxP3 (ebioscience 53‐4776) were added to the samples in different combinations (Table ) to allow determination of subset‐specific activation and cytokine production and were incubated at 4°C for 20 minutes.

Techniques: Staining, Marker, Activation Assay

Journal: Developmental and comparative immunology

Article Title: Identification and characterization of equine blood plasmacytoid dendritic cells.

doi: 10.1016/j.dci.2016.08.005

Figure Lengend Snippet: Fig. 1. Phenotype of Flt3þ cells in equine PBMC. (A) Gating strategy to remove doublets and include FSChigh/SSChigh large cells. A total number of 250,000 cells were acquired. (B) Within the single, large cell gate, based on the fluorescence minus one (FMO) control, a gate on Flt3þCD14 cells was set. (C) Identification of three putative DC subpopulations based on MHCII and CADM-1 expression within Flt3þCD14 gated cells. (D) Histograms showing the expression levels of CD172a, CD4 and CD13 in P4 (red; pDC), P5 (blue; cDC2) and P6 (orange; cDC1) compared to PBMC (grey). Data are shown for one representative animal out of three (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Other surface markers were stained using the following antibodies: anti-equine CD4 (clone CVS4; cat no MCA1078, Bio-Rad; Antibody Register AB_321274), anti-equine CD13 (clone CVS19; cat no MCA1084GA, Bio-Rad; Antibody Registry: AB_321308), antiequine MHCII (clone CVS20; cat no MCA1085, BioRad; Antibody Register AB_321618), anti-equine CD14 (clone 105; https:// courses2.cit.cornell.edu/wagnerlab/research/reagents.htm; Kabithe et al., 2010), anti-bovine CD172a (clone HR-DH59B; cat no HR-BOV2049; Monoclonal Antibody center, Washington State University; Pullman WA, USA) showing cross-reactivity with equine cells (M erant et al., 2009).

Techniques: Control, Expressing

Journal: Journal of neuroimmunology

Article Title: Increased maternofoetal transfer of antibodies in a murine model of systemic lupus erythematosus, but no immune activation and neuroimmune sequelae in offspring.

doi: 10.1016/j.jneuroim.2022.577927

Figure Lengend Snippet: Fig. 1. Total IgG2a levels of either the IgG2A or IgG2C allotype in BXSB/MpJ, C57BL/6 J and DBA/2JRj dams. (A) Total IgG2A in BXSB/MpJ (n = 4), C57BL/6 J (n = 6), and DBA/2JRj (n = 8), controls. Significantly higher levels of IgG2A were observed in DBA/2JRj controls compared to both C57BL/6 J (p = 0.0013) and BXSB/MpJ (p = 0.0036). (B) Total IgG2C in BXSB/MpJ (n = 4), C57BL/6 (n = 6), and DBA/2JRj (n = 8) controls. IgG2C levels in BXSB/MpJ and C57BL/6 J controls were significantly greater compared to DBA/2JRj (p = 0.0292 and p = 0.0005, respectively). Statistical significance based on multiplicity- adjusted p-value determined using one-way ANOVA with Tukey's test for multiple com parisons. * = p < 0.05, ** = p < 0.01, *** = p < 0.001. n = sample size.

Article Snippet: Wells were next incubated 2 h at RT with biotinylated Goat anti-mouse antibody, diluted in TBS/Tw to 1 μg/ml, according to the desired antibody or allotype measured: Goat anti-mouse IgG2A (Southern Biotech, Cat.: 1083–08), Goat anti-mouse IgG2C (Southern Biotech, Cat.: 1077–08) or Goat anti-mouse Ig (Southern Biotech, Cat.: 1010–08).

Techniques:

Journal: Journal of neuroimmunology

Article Title: Increased maternofoetal transfer of antibodies in a murine model of systemic lupus erythematosus, but no immune activation and neuroimmune sequelae in offspring.

doi: 10.1016/j.jneuroim.2022.577927

Figure Lengend Snippet: Fig. 5. Anti-dsDNA antibody levels in BXSB/MpJ, C57BL/6 J, and DBA/2JRj dams. (A) Anti-dsDNA IgG2A in BXSB/MpJ (n = 4), C57BL/6 J (n = 5), and DBA/2JRj (n = 5) controls. Anti-dsDNA IgG2A was hardly detectable in all groups. (B) Anti-dsDNA IgG2C in BXSB/MpJ (n = 4), C57BL/6 J (n = 5), and DBA/2JRj (n = 8) controls. Between all groups, levels of anti- dsDNA IgG2C were diminutive and compa rable to the DBA/2JRj controls. Statistical significance was tested by one- way ANOVA with Tukey's test for multiple comparisons. No significant differences were detected. AU = arbitrary units, n = sample size.

Article Snippet: Wells were next incubated 2 h at RT with biotinylated Goat anti-mouse antibody, diluted in TBS/Tw to 1 μg/ml, according to the desired antibody or allotype measured: Goat anti-mouse IgG2A (Southern Biotech, Cat.: 1083–08), Goat anti-mouse IgG2C (Southern Biotech, Cat.: 1077–08) or Goat anti-mouse Ig (Southern Biotech, Cat.: 1010–08).

Techniques: